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KMID : 0984720030350060377
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Infection and Chemotherapy
2003 Volume.35 No. 6 p.377 ~ p.384
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Molecular Diversity of vanA Gene Cluster in Vancomycin-resistant Enterococci Isolated in One University Hosipital
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Kim Jung-Min
Lee Ji-Hyang
Yoon Jeong-Joong
Lee Nam-Yong
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Abstract
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Background : The vanA gene is the most frequently encountered gene among isolates, causing vancomycinresistant Enterococci (VRE) infections in humans, and it is part of the transposable element Tn 1546. Knowledge of the diversity of Tn 1546 is important to distinguish between the dissemination of a single VRE clone and the transmission of a particular Tn 1546 type through a genetically divergent population of enterococci. Recently, we studied molecular diversity of Tn 1546related elements in enterococci isolated in one hospital to facilitate understanding of the molecular epidemiology of vancomycin resistance.
Methods : Nineteen VanAtype VRE clinical isolates, collected in one university hospital during 1997 and 1999, were investigated for characteristics such as antibiotic resistance, structure of vanA gene cluster and genomic DNA type by means of antibiotic susceptibility test, PCR amplified length polymorphism of vanA gene cluster and pulsefield gel electrophoresis (PFGE), respectively.
Results : Nine (A, B1 to B5, C, D and E) different vanA gene cluster types were identified. Three isolates were grouped into vanA gene cluster type A, similar to that of Tn 1546 prototype, and twelve isolates were grouped into type B that has an insertion of IS 1216V at vanXY intergenic region. Type B was further subdivided into 61 to B5 according to the size variation of vanXY intergenic region, which was resulted from the insertion of IS1216V and deletions associated with the insertion. Both vanY and vanZ were deleted in three isolates, suggesting that these genes are not essential for vancomycin resistance. Notably, three E. faecalis and three E. faecium strains isolated during the same period were shown to carry the same vanA gene cluster, showing intergenic transmission of vancomycin resistance.
Conclusion : The presence of variable types of vanA gene cluster among VRE strains isolated in one hospital suggests that several evolutionary changes of vanA gene clusters have occurred during the horizontal spread of resistance gene in the hospital environment. This approach may be useful for monitoring the evolution of VanA resistance.
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KEYWORD
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VanA-type vancomycin-resistant enterococci, VanA gene cluster, PFGE
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